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Following overnight TEV digestion at 4 C, both MBP and GST elution were subjected to Strep-Tactin Sepharose resin, washed with wash buffer and eluted with wash buffer supplemented with 10 mM desthiobiotin

Refrigerator vs Freezer for Unopened Vials For vials that will be used within the next few weeks to months, standard refrigerator storage at 2 to 8 degrees Celsius is adequate

doi: 10.1007/s00134-015-3771-8 75 GoldPW

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